Bovine Anti-Mullerian Hormone (MIS;AMH) ELISA Kit

Cat.#:

JL16387

Regulatory Status:

RUO

Alternative:

MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

78.12-5000pg/mL

Sensitivity:

34.76pg/mL

Specificity:

This kit recognizes Bovine MIS;AMH in samples. No significant cross-reactivity or interference between Bovine MIS;AMH and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Developmental Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| MIS;AMH

Anti-Müllerian hormone (AMH), also known as Müllerian-inhibiting hormone (MIH), is a glycoprotein hormone structurally related to inhibin and activin from the transforming growth factor beta superfamily, whose key roles are in growth differentiation and folliculogenesis.AMH is a dimeric glycoprotein with a molar mass of 140 kDa.The molecule consists of two identical subunits linked by sulfide bridges, and characterized by the N-terminal dimer (pro-region) and C-terminal dimer.AMH binds to its Type 2 receptor AMHR2, which phosphorylates a type I receptor under the TGF beta signaling pathway.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	5000	2500	1250	625	312.5	156.25	78.12	0
OD	0.13	0.19	0.32	0.56	0.8	1.18	1.47	2.04

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Bovine MIS;AMH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-102	95
Plasma(n=8)	94-107	104
Cell Culture Supernates(n=8)	96-112	104

| Linearity

Samples were spiked with high concentrations of Bovine MIS;AMH and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	82-95	88-96	90-110
1 : 2	Average (%)	91	91	97
1 : 4	Range (%)	89-103	87-110	105-115
1 : 4	Average (%)	94	98	109